Week 14 Research (Nov 28 - Dec 4)
12/02/11 (Crystallization Workshop) Hands On: Solving a crystal structure with molecular replacement and structure refinement using CCP4: Visualization using Coot
11/28/11 (Crystallization Workshop) Lecture: Phasing and the Refinement of crystal structures.
Week 13 Research (Nov 21 - Nov 27)
Week 12 Research (Nov 14 - Nov 20)
11/16/11 (Crystallization Workshop) Hands On: Data Processing
11/14/11 (Crystallization Workshop) Lecture: Introduction to Crystal Symmetry & Data Processing
Week 11 Research (Nov 7 - Nov 13)
11/11/11 (Crystallization Workshop) Hands On: Crystal Data Collection Workshop
11/08/11 - Bio Symposium
11/07/11 (Crystallization Workshop) Lecture: Crystal Diffraction
11/07/11 - Submitted compounds to order
Week 10 Research (Oct 31 - Nov 6)
11/01/11 (Crystallization Workshop) Hands On: Used the Crys-Cam Robotic Viewing Instrument to visualize the crystallization plates set up with the Phoenix machine. Also set up hanging drop experiments manually.
Week 9 Research (Oct 24 - Oct 30)
10-24-11 (Crystallization Workshop) Lecture: Fundamentals of Crystallization, A Survey of Methods, and an Introduction to the Phoenix Crystallization Robot.
10-26-11 (Crystallization Workshop) Hands On: Set up crystallization experiments using the Phoenix robot
Week 8 Research (Oct 17 - Oct 23) Week 7 Research (Oct 10 - Oct 16) Week 6 Research (Oct 3 - Oct 9) Week 5 Research (Sept 26 - Oct 2) Week 4 Research (Sept 19 - Sept 25) Week 3 Research (Sept 12 - Sept 18)
9/12/11 - LSAMP Conference
Week 2 Research (Sept 5 - Sept 11) Week 1 Research (Aug 29 - Sept 4)
SUMMER RESEARCH BELOW
Figure 1. LB Agar + Amp plate with colony growth of DNA plasmid pGFP. Plasmid A plate contains 1ng of plasmid. About 15 colonies grew overnight.
Figure 2. LB Agar + Amp plate with colony growth of DNA plasmid pGFP. Plasmid B plate contains 5ng of plasmid. 125 colonies grew overnight.
Figure 2. LB Agar + Amp plate with colony growth of DNA plasmid pGFP. Plasmid C plate contains 5ng of plasmid.
Lane 1 Skip Lane 2 1kb DNA ladder Lane 3 Uncut plasmid Lane 4 EcoRi Lane 5 PvuII Lane 6 EcoRi+PvuII Error: gel was left on for too long on the voltage. The material on the lanes slid off.
Lane 1 Skip Lane 2 1kb DNA ladder Lane 3 Uncut plasmid Lane 4 EcoRi Lane 5 PvuII Lane 6 EcoRi+PvuII
(Lane 1) Skipped (Lane 2) 100bp Marker (Lane 3) Sample A (Lane 4) Sample B (Lane 5) Sample C (Lane 6) Sample D (Lane 7) 100bp Marker (Lane 8) Sample A (Lane 9) Sample B (Lane 10) Sample C (Lane 11) Sample D (Lane 12) Skipped Gel Shared Joey: Lanes 1-6 J.C. : Lanes 7-12
Lane 1: Skipped Lane 2: 100bp Marker Lane 3: Sample A -6ng plasmid 0ul MgCl2 Lane 4: Sample B - 6ng Plasmid 1.5ul MgCl2 Lane 5: Sample C - 6ng plasmid 2ul MgCl2 lane 6: Sample D - 6ng Plasmid 4ul MgCl2 Lane 7: Sample E - 6ng Plasmid 6ul MgCl2 Lane 8: Sample F - No Plasmid 1.5ul MgCl2 (1st Gel Attempt using Original Protocol)
Joey's Research
Joey - any new results? _ put in reverse chronological order. - Dr. B
06/14/2012
Week 14 Research (Nov 28 - Dec 4)
12/02/11 (Crystallization Workshop) Hands On: Solving a crystal structure with molecular replacement and structure refinement using CCP4: Visualization using Coot
11/28/11 (Crystallization Workshop) Lecture: Phasing and the Refinement of crystal structures.
Week 13 Research (Nov 21 - Nov 27)
Week 12 Research (Nov 14 - Nov 20)
11/16/11 (Crystallization Workshop) Hands On: Data Processing
11/14/11 (Crystallization Workshop) Lecture: Introduction to Crystal Symmetry & Data Processing
Week 11 Research (Nov 7 - Nov 13)
11/11/11 (Crystallization Workshop) Hands On: Crystal Data Collection Workshop
11/08/11 - Bio Symposium
11/07/11 (Crystallization Workshop) Lecture: Crystal Diffraction
11/07/11 - Submitted compounds to order
Week 10 Research (Oct 31 - Nov 6)
11/01/11 (Crystallization Workshop) Hands On: Used the Crys-Cam Robotic Viewing Instrument to visualize the crystallization plates set up with the Phoenix machine. Also set up hanging drop experiments manually.
Week 9 Research (Oct 24 - Oct 30)
10-24-11 (Crystallization Workshop) Lecture: Fundamentals of Crystallization, A Survey of Methods, and an Introduction to the Phoenix Crystallization Robot.
10-26-11 (Crystallization Workshop) Hands On: Set up crystallization experiments using the Phoenix robot
Week 8 Research (Oct 17 - Oct 23)
Week 7 Research (Oct 10 - Oct 16)
Week 6 Research (Oct 3 - Oct 9)
Week 5 Research (Sept 26 - Oct 2)
Week 4 Research (Sept 19 - Sept 25)
Week 3 Research (Sept 12 - Sept 18)
9/12/11 - LSAMP Conference
Week 2 Research (Sept 5 - Sept 11)
Week 1 Research (Aug 29 - Sept 4)
SUMMER RESEARCH BELOW